cell signaling cx43 rabbit  (Cell Signaling Technology Inc)

Journal: BioMed Research International

Article Title: Cardiac Electrophysiological Alterations in Heart/Muscle-Specific Manganese-Superoxide Dismutase-Deficient Mice: Prevention by a Dietary Antioxidant Polyphenol

doi: 10.1155/2014/704291

Figure Lengend Snippet: Connexin 43 (Cx43) protein levels in the hearts of control and H/M- Sod2 −/− mice. (a) Cx43 levels in the hearts of control and H/M- Sod2 −/− mice with and without AP intake. Western blot analysis of Cx43 protein extracted from hearts was carried out using antibodies against Cx43 and actin, n = 4. (b) Immunostaining with Cx43-specific antibodies of the left ventricular sections of control and H/M- Sod2 −/− mice. Cx43 protein levels in the H/M- Sod2 −/− heart relative to the control heart, and chronic 0.1% AP intake partially improved Cx43 expression. (c) Normalized fluorescence intensity for Cx43 immunostaining of the left ventricular sections obtained from H/M- Sod2 −/− mice. The fluorescence intensity level was normalized to that determined in the left ventricular sections of control mice. Values are mean ± SEM, n = 4, * P < 0.05.

Article Snippet: Proteins were separated by SDS-PAGE, transferred to a PVDF membrane and probed with specific antibodies to connexin 43 (Cx43) (1 : 1000; Cell Signaling, Danvers, MA) and actin (1 : 2500; Sigma, St. Louis, MO).

Techniques: Control, Western Blot, Immunostaining, Expressing, Fluorescence